Current Issue : October - December Volume : 2020 Issue Number : 4 Articles : 6 Articles
We examined the vascularity of mammary Paget disease histologically to confirm the increased blood\nflow observed previously by clinical imaging. The relationships among blood vessel density (BVD), histopathological\nparameters of blood flow in the nipple, and the expression of angiogenic factors such as basic fibroblast growth\nfactor (bFGF) and vascular endothelial growth factor A (VEGFA) were examined.\nMethods: We calculated the average CD34-positive BVD and podoplanin (D2-40)-positive lymphatic vessel density\n(LVD) and the proportion of proliferating of endothelial cells in 14 Paget disease, 3 dermatitis biopsy, and 14 agematched\ncontrol cases. As a parameter related to blood flow in the nipple, the total CD34-positive blood vessel\nlumen area relative to the entire nipple area was measured in each Paget disease and control case using an\nautomated image analysis system. Immunohistochemical expression of bFGF and VEGFA in Paget cells was also\nexamined.................
Without early recognition and Kasai procedure, biliary atresia (BA) results in liver cirrhosis and leads to\neither transplantation or death at a young age. We aimed to characterize the liver histopathological findings for\nprediction of cirrhosis and survival in BA patients after Kasai surgery.\nMethods: We retrospectively reviewed all histopathological results for BA patients who underwent liver biopsy\nduring Kasai surgery from August 2012 to December 2018 in Dr. Sardjito Hospital, Yogyakarta, Indonesia.\nResults: Fifty infants with BA were ascertained in our study, of whom 27 were males and 23 were females. The\nmedian age of Kasai procedure was 102.5 days (interquartile range (IQR), 75.75-142.25 days). There were 33 (66%)\nand 17 (34%) BA patients with and without liver cirrhosis, respectively, while the overall survival was 52%. The\npatients with a severe bile duct proliferation, severe .......................
Expression of forkhead box P3 (FOXP3), a key regulator of T-cell function, in the tumor immune\nmicroenvironment is related to survival in classic Hodgkin lymphoma (CHL). Vitamin D receptor (VDR), a\ntranscription factor agonists have been shown to induce FOXP3 expression in T-cells and enhance recruitment of\nthese cells to the inflammatory sites. VDR expression is CHL has been described. However, there is no data on\nexpression of VDR in context of quantity of FOXP3 positive cells in CHL.\nMethods: We examined and correlated immunohistochemical expression of VDR and FOXP3 along with clinical\nand pathology findings in 29 cases of CHL.\nResults: VDR was expressed in Hodgkin Reed-Sternberg (HRS) cells and background lymphocytes and FOXP3 was\nexpressed in background lymphocytes. 82% of CHL cases, regardless of the subtype, expressed VDR and in majority\nof the cases, VDR expression was directly proportional to the quantity of FOXP3 expressing lymphocytes in the\ntumor microenvironment. In cases with higher clinical stage.........................
This study evaluated the predictive power of Atyp.C (a parameter of UF-5000 flow cytometer) for\npatients with a suspected diagnosis of urothelial carcinoma.\nMethods: We analyzed 163 urine specimens from 128 patients with suspected urothelial carcinoma using a fully\nautomated fluorescence flow cytometry analyzer (UF-5000) and evaluated its performance on identifying atypical/\nmalignant urothelial cells. From January 1, 2019 to April 4, 2019, all consecutive specimens for urinary\ncytopathology were enrolled.\nResults: Of the specimens with urinary cytopathology, 67 specimens (41.1%) revealed abnormal findings in\ncytology analysis. Among them, 20 specimens (12.3%) were diagnosed as atypical urothelial cells, 26 specimens\n(16.0%) as suspicious for malignancy (S-malignancy), and 21 specimens (12.9%) as confirmed malignancy. The UF-\n5000 findings were positive in 59 specimens (36.2%); therefore, the agreement with cytopathology was 73.0%.\nUsing follow-up histologic diagnosis of urothelial carcinoma with or without urinary tract cytology (UTCy) as a\nreference standard (suspicious and confirmed malignancy were the positive criteria for UTCy), the sensitivity was\n59.0%, specificity was 82.1%, positive predictive value was 75.0%, negative predictive value was 68.8%, and the\nagreement was 71.1%.\nConclusions: It is worth knowing and reporting that the Atyp.C assay may be used as an accessory test for patients\nwith suspected urothelial carcinoma, based on its ability to identify high-risk patients who might need closer\nfollow-up or additional medical treatment....
Helicobacter pylori (H. pylori) can cause gastritis, peptic ulcer diseases and gastric carcinoma.\nEndoscopy as the gold standard method of diagnosis is an invasive procedure that might not be\nsuitable in all scenarios. Therefore, this first study in Jordan aimed to assess the non-invasive 13C urea\nbreath test (UBT) and stool antigen test for diagnosis of H. pylori infection and the successfulness of\neradication therapy as alternatives for endoscopy. Hence, a total of 30 patients attending the endoscopy\nunits at Alkarak teaching hospital were asked to complete a questionnaire with demographic and\nclinical data. They were then tested for H. pylori using 13C UBT and H. pylori stool antigen before\nhaving endoscopy. Another 30 patients who were positive for H. pylori by endoscopy were tested\nusing both tests 6 weeks post eradication therapy. Results showed that the rate of H. pylori detection\nusing endoscopy was 56.7% (17/30). Heartburns....................
Yolk sac tumor (YST) is a germ cell neoplasm that arises predominantly in the gonads, but can also\nderive from somatic neoplasms in extragonadal locations. These latter cases have been documented in several\norgans, although reports from the urinary tract are limited. To our knowledge, this is the first report of a bladder\nurothelial carcinoma with a predominant component of YST differentiation.\nCase presentation: We present a unique case of a 76-year-old man with a recurrent urinary bladder tumor, initially\ninterpreted as a high grade urothelial carcinoma with glandular differentiation. In the recurrent tumor, diverse\nhistological patterns were identified, including glandular, hepatoid and sarcomatoid. This tumor showed positivity\nfor AFP, GLP3 and SALL4, and negativity for CK7 and EMA. Fluorescent in situ hybridization study showed a\npolysomic pattern of chromosome 12. All these findings led to the final diagnosis of a YST derived from urothelial\ncarcinoma.\nConclusions: YST differentiation should be considered in the differential diagnosis of a high grade urothelial\ncarcinoma, particularly when glandular and other unusual patterns are observed....
Loading....