Current Issue : April-June Volume : 2012 Issue Number : 2 Articles : 1 Articles
The present investigation was conducted to develop a protocol for rapid callus induction and its proliferation. The leaflet, cotyledon, epicotyl and hypocotyl explants of Withania somnifera were cultured on to SH (Shenk and Hildebrandt) supplemented with different concentration and combinations of Auxin (2, 4-D and IBA) alone and combination with cytokinins (BAP and Kin). The highest callus induction (93.33 %) observed on SH medium supplemented with 1.5 ppm 2, 4-D. highest Green mass was found on SH+2, 4-D (2.0 ppm)+ BAP (1.0) ppm and dry mass also found on SH+ 2,4-D (2.0 ppm) + BAP (1.0 ppm)....
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