Current Issue : January - March Volume : 2013 Issue Number : 1 Articles : 10 Articles
The present study is conducted to evaluate the antibacterial and wound healing activities of leaves of plants Rosa indica (Rosaceae) by Invitro and in vivo model studies. Studies on the antibacterial activity of 70% ethanol and methanol extract of Rosa indica (pink rose, orange rose) leaves were conducted using agar well diffusion method and on wound healing activity. 70% ethanol and methanol extract of Rosa indica leaves (pink rose, orange rose) conducted using Excision wound model . The micro organism used were Staphylococcus aureus,Bacillus subutilis, Escheria coli and animals used were Albino rat (wistar strain). The results showed that 5mg/ml showed moderate zone of inhibition against staphylococcus aureus and Escheria coli, maximum zone of inhibition were produced against them at 20mg/ml. Bacillus subtilis was resistant to both ethanolic and methanolic extracts.(Table1). Both ethanolic and methanolic extracts of orange rose variety were only effective against staphylococcus aureus at 20mg/ml and In wound healing study the 5mg/ml ethanolic extract of leaves of pink rose has highest wound healing activity .Also 5mg/ml methanolic extract of leaves of pink rose has remarkable wound healing activity in compare to control and standard.On the other hand 5mg/ml ethanolic extract of leaves of orange rose has better wound healing activity than standard povidone iodine.The above result revealed that the ethanolic pink rose leaves extract has remarkable anti bacterial and wound healing potency and appear to justify the traditional use of Rosa indica.....
The present study evaluated the In vitro anticataract and antioxidant activities of commercially available flavonoids against glucose-induced cataractogenesis using goat lenses. Transparent isolated goat lenes were incubated in artificial aqueous humor and divided into six experimental groups. The compounds at a dose of 100 μg/ml were incubated simultaneously with glucose (55 mM) for a period of 72 h. Vitamin E (100 μg/ml) was used as the standard drug. At the end of the incubation, levels of various biochemical parameters such as protein content, malondialdehyde (MDA), lipid hydroperoxides (LH), Cu2+-induced lipoprotein diene formation, Ca2+ATPase and enzymatic antioxidants like catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GSSH) and non-enzymatic antioxidant like reduced glutathione (GSH) were measured in the lens homogenate. Incubation with glucose produced a mature cataract and there was a significant increase in protein content, Ca2+ATPase, and a decrease in LH, MDA, Cu2+-induced lipoprotein diene formation and enzymatic and non-enzymatic antioxidants when compared to normal control. All the compounds exhibited a dose dependent aldose reductase inhibitory activity when compared to the standard epalrestat (IC50 = 0.75 ± 0.07 µg/ml). Our results indicated that simultaneous incubation of the plant extracts prevented the preoxidative damage caused by glucose, which is evidenced from the improved antioxidant potential. The selected compounds protected the lens against glucose-induced oxidative damage which might be helpful in delaying the progression of cataract....
The flowers of Musa sapientum L. (MS) commonly known as ‘Banana Flowers’ are reported to be used in Indian folk medicine for the treatment of diabetes mellitus. But the probable mechanisms of antidiabetic activity are yet to be studied. The present study was designed to evaluate the antihyperglycemic activity of banana (Musa sapientum) flowers and development of suitable dosage form. The air dried powder sample of flower of Musa sapientum was extracted with ethanol and water by maceration method and subjected for phytochemical analysis. The extracts were analyzed by TLC which shown the presence of gallic acid. The quantitative estimation of gallic acid from extract was done by High Performance Thin Layer Chromatography (HPTLC). The antidiabetic potential of the extracts was tested using Oral Glucose Tolerance Test (OGTT) in normal rats. In OGTT, there was a significant reduction in glucose level of animals treated with aqueous and ethanolic extract at the dose of 200 mg/kg. In vitro assays were also performed for estimation of inhibition of α-glucosidase and α-amylase. IC50 values were calculated and inhibition efficiencies of aqueous and ethanolic extract were compared. The results have shown that both the extracts have good potential in management of diabetes. The human equivalent dose was calculated from OGTT results and tablet of 750 mg was formulated each containing 500mg aqueous extract of MS with optimized formula of excipients. The formulation was characterized and evaluated for physicochemical stability, and the gallic acid content in the table was assayed by HPTLC....
Diabetes mellitus is considered one of the major threats to health and well being of human race in the twenty first century. The number of young and middle aged people getting affected with diabetes is alarmingly high. This higher rate of incidence can be attributed to the sedentary lifestyle, physical inactivity and unhealthy food habits. Better health care facilities have resulted in long lifespan, age related diseases and complications. People get diagnosed with diabetes and they tend to live long with the associated complications. It is important that the complications do not become fatal to the individual and the diabetic must be allowed to live a near normal life with good glycemic control.Prevention of development of complications must be given prime importance. Skilful and wise administration of insulin, oral hypoglycemic agents; that are widely used in allopathic medicine along with the time tested and trial tested herbal medicines give a lot of hope and promise in this direction. Traditional Chinese medicine gives an array of herbs that can be utilized for the wellbeing of diabetics. Use of herbs may reduce the adverse effects of allopathic drugs, provided; more attention is given to herb- drug interactions and contraindications to treatment with herbs. Healthcare professionals’ knowledge, attitudes and practices regarding the use of herbs are of great importance. The medical fraternity as well as the patients must be educated regarding the right approach to diabetes and its control....
Diabetic nephropathy progressed to end-stage renal disease (ESRD) is found in type 1 or type 2 diabetes. Oxidative stress is one of the precipitation factors in diabetic nephropathy. The phenolic derivative maker compound Protocatechuic acid (PCA) shows variety of therapeutic activities. Literature data shows that preclinical evaluation of some activities is still remaining. PCA claims to possess activity against renal complications viz. nephropathy, renal ischemia. The aim of present work was to do screening of PCA against renal complications like diabetic nephropathy by using animal model like alloxan induced diabetic nephropathy in which PCA showed significant activity of inhibition of diabetic nephropathy through inhibition of oxidative stress in experimental animals....
The present work was performed to evaluate the effects of aqueous stem bark extract of Ailanthus excelsa (AESE) for anti-rheumatoid activity. Three doses of AESE (100, 200 & 400 mg/kg orally) were selected. Arthritis was induced by injecting 0.1 ml (0.1% w/v) of complete Freund’s adjuvant in subplanter region of left hind paw of Wistar albino rats. Different paramaters like paw edema (inflammation), body weight, ankle joint diameter, mechanical hyperalgesia, hematological studies, biochemical studies, spleen weight, radiological and histological analysis of bone damage were assessed in Freund’s adjuvant induced paw inflammatory model. Methotrexate (0.75 mg/kg, p.o) was the standard reference drug. Paw volume changes were estimated using plethysmometer and Randall-selieto apparatus used to determine the mechanical hyperalgesia. All dose levels of Ailanthus excelsa aqueous stem bark extract (AESE) showed significant and dose-dependent anti-rheumaroid effects compared to arthritic control group. Altogether these results suggest that the aqueous stem bark extract of Ailanthus excelsa could be considered as a potent anti-rheumatoid agent....
Antihepatotoxic activity of Cassia tora L. (Leguminoseae) was evaluated using in-vivo screening of ethanoilc extract and its ethyl acetate and butanoilc fractions. Histopathological studies were also carried out. The biochemical parameters selected were estimation of SGOT, SGPT and SAPT. The enzyme levels compared with control showed significant protection. The protective percentage was maximum for butanolic fraction which was 82.92%, 83.49% and 75.8% respectively. In histopathological finding of butanolic fraction also a remarkable recovery was observed with distinct hepatocytes, prominent nucleus and nucleolus. There were no lesions. The data justify ethnopharmacological application of Cassia tora....
In the present study we investigated the effect of hydroalcoholic extract of fruit of Terminalia chebula (TCE) in alleviating the diabetic impotency in rats. Male wistar albino rats were classified into two groups as normal control and diabetic group. Normal control group rats were injected with distilled water (1ml/kg) while in diabetic group, diabetes mellitus was induced by intraperitoneal injection of Alloxan monohydrate ( 120 mg/kg) freshly dissolved in normal saline solution.21 days after diabetes induction, the rats in diabetic group were individually tested for sexual potency by pairing them with oestrus female rat. Diabetic rats showed a significant decline in the sexual potency as compared to the normal control groups. Following this, the diabetic rats were divided into five groups and treated orally as: diabetic control group with distilled water 1 ml/day, TCE-200 group with TCE 200 mg/kg, TCE-400 group with TCE 400 mg/kg, TCE-800 group with 800 mg/kg and standard group with sildenafil citrate 5 mg/kg for 21 days. Their sexual behavior was evaluated on 14 th and 21st day of respective treatment. The study revealed dose dependent improvement in all the parameters of sexual behavior against diabetic control group, reflecting increased potency. These results suggest that TCE have beneficial activity against diabetic impotency in rats.This observed activity of TCE may be due to the presence of phytoconstituents like tannins , alkaloids, saponins, flavonoids. Further studies required to determine the exact molecular mechanism of action of Terminalia chebula....
Important plant foods in traditional diet such as herbs, onion and beans have high phenolic phytochemicals. Among these plant foods Allium species, onions and garlic have been traditionally used for a large range of purposes. On the basis of traditional claim for Musa species and reported activities the cummulative account is formulated. Musa Paradisiaca, Musa sapientum, and Musa cavendish a higly growing fruits in the India. This review include the pharmacological activities of different Musa species along with the phytohemical constitutents responsible for the specific activity....
From time immemorial plants have been used as a source for health care by human society. In this preclinical study, three polyherbal appetizers formulation churna were prepared using medicinal plants such as Zingiber officinale, Carum carvi, Terminalia chebula, Piper nigrum, Plectranthus Amboinicus, Eugenia Caryophyllus, Foeniculum vulgare, Coriandrum sativum, Mentha piperita. The present study is undertaken to investigate the effects of appetizers on food consumption, weight gain, and leptin levels in Wistar rats. The polyherbal appetizer churna were prepared in lab scale and administered to groups of rats. The Fasting leptin levels of the rats ranged from 1.07 to 1.11 ng/ml, while weights were in the range of 92.2 to 122.4 gm. Decreased (18.01, 13.63, and 11.81%) leptin levels following the consumption of three appetizers indicated their appetizing effect. Weight gain after 15 days was 4.37% in the control group whereas 55.17, 43.20 and 32.77% in rats fed with three different polyherbal appetizer churna, respectively. \nKeywords: Appetizer, Food Consumption, Leptin, Churna...
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